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Prof. Sheng Qin's Research Group Advances in 2017-2018
文章作者: 发布时间: 2018-06-29 访问次数: 576

Soil salinity is one of the major stresses that limit global agricultural productivity, affecting more than 45 million ha of irrigated land worldwide. Some plants, termed halophytes, have evolved complex mechanisms to adapt to saline stress. Plant-associated microorganismsare considered a key determinant of plant health and growth. However, little information is available regarding the composition and ecological function of the roots and leaves bacterial microbiota of halophytes. In our previous study, our group isolated many endophytic and rhizosphere bacteria from coastal halophyte Limonium sinense, and obtained some potential PGP strains using seedlings inoculation on MS medium under salt stress (Qin et al., Plant Soil, 2014, 374:753–766). Three strains of the genera Streptomyces, Bacillus and Arthrobacter could significantly promote host growth under salt stress. Recently, plant growth-promoting effect using pot experiments and plant physiological analysis by two beneficial endophytes of the genera Streptomyces and Bacillus isolated from halophyte Limonium sinense were studied (Qin et al., Plant Soil, 2017, 416:117–132). The genomes of the strains were also sequenced and analyzed (Wang et al., Journal of Biotechnology, 2017, 260: 38-41). These studies are helpful to understand how endophytes help host plants grow in salt marshes environment. Using both culture-dependent and culture-independenttechniques, we characterized the bacterial communities of the roots, leavesas well as the rhizosphere and bulk soils of the coastal halophyte Limonium sinense in Jiangsu Province, China. We found that Glutamicibacter was the most dominant genus. All Glutamicibacterisolates showed multiple potential plant growth promotion traits, and tolerated high concentration of NaCl and wide pH range. Interestingly, further inoculation experiments showed that the new species Glutamicibacter halophytocolaKLBMP 5180(Feng et al., Int J Syst Evol Microbiol, 2017, 67(5): 1120-1125) isolated from root tissue significantly promoted host growth under NaCl stress. Indeed, KLBMP 5180 inoculation increased the concentrations of total chlorophyll, proline, anti-oxidative enzymes, flavonoids, K+, and Ca2+ in the leaves; concentrations of malondialdehyde (MDA) and Na+ were reduced. Transcriptome analysis revealed that pathways related to phenylpropanoid and flavonoid biosynthesis, and ion transport and metabolism might play more important roles in host salt-stress tolerance induced by KLBMP 5180 inoculation compared to non-inoculated leaves. Our results provided novel insights into the complex composition and function of the bacterial microbiota of coastal halophyteL. sinense, and suggested that halophytes might recruit specific bacteria to enhance their tolerance of harsh environments (Qin et al., Appl Environ Microbiol, 2018, 84:e01533-18). Besides, we assessed actinobacterial diversity from eight coastal salt marsh rhizosphere soils from Jiangsu Province, China, using culture-based and 16S rRNA gene high throughput sequencing methods. Actinobacterial sequences represented 2.8%–43.0% of rhizosphere bacterial communities, as determined by HTS technique. The 92 representative cultured strains comprised seven suborders, 12 families, and 20 genera that included several potential novel species (Dinget al., Int J Syst Evol Microbiol, 2018, 68:192–197). Two strains were selected for inoculation of wheat seeds grown under salt stress. Our study demonstrates that coastal salt marsh rhizosphere soils harbor a diverse reservoir of Actinobacteria that are potential resources for the discovery of novel species and functions (Gong et al., Syst Appl Microbiol, 2018, 41:516–527).


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